THE ALKALINE HYDROLYSIS OF EGG ALBUMIN
نویسندگان
چکیده
منابع مشابه
The Alkaline Hydrolysis of Egg Albumin
Following the discovery of Kossel and Weiss (1) that many of the constituent amino acids are racemized when proteins are hydrolyzed by alkali, the use of alkaline hydrolysis in the analysis of proteins has been limited to the determination of amino acids which are unstable in strong acid. There is little information available as to the extent and completeness of hydrolysis as a function of [OH-...
متن کاملThe Stages of the Peptic Hydrolysis of Egg Albumin
1. Most of the products of the peptic hydrolysis of albumin, about 85 per cent of the total N, are primary in the sense that they arise directly from the protein molecule, and undergo no further hydrolysis. 2. A slow secondary hydrolysis, involving about 15 per cent of the total N, occurs in the proteose and simpler fractions primarily split off. 3. Acid metaprotein in peptic hydrolysis arises ...
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The interaction of bovine serum albumin ( BSA) and egg albumin with water solublephthalocyanine, cobalt (ΙΙ) 4, 4′ , 4′′, 4′′′- tetrasulfophthalocyanine ( CoTSPc) , has been studiedby the UV- Vis method at pH 7.0 and five different temperatures 20, 25, 30, 35 and 40°C. Theformation constants have been elucidated by using spectrophotometric titration and computerSQUAD program data refinement. Th...
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A faster reaction of the cation is expected for the compounds with a free amino group in the acyl moiety. Such an increase was observed for Ala-Ala-pnitroan~lide b.ut not with Ala-p-nitroanilide. Fischer · et al. concluded that a larger inc-rease in k1 with the latter compound was compensated by a reductionin k2/ki. so that the observed k1k2/(k~1 + k2) term remains the same. This assumption of ...
متن کاملThe Sulfhydryl Groups of Egg Albumin
1. 1 cc. of 0.001 M ferricyanide, tetrathionate, or p-chloromercuribenzoate is required to abolish the SH groups of 10 mg. of denatured egg albumin in guanidine hydrochloride or Duponol PC solution. Both the nitroprusside test and the ferricyanide reduction test are used to show that the SH groups have been abolished. 2. 1 cc. of 0.001 M ferrocyanide is formed when ferricyanide is added to 10 m...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1942
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)45074-0